Odor Map

Data

Name c-fos glomerular activation patterns (a comparison for H-2b and H-2k male urine odors)

Description Nonestrous

Species Mouse

Strain BALB/C (H-2d)

Sex Female

Age 12 to 20 weeks

Weight

Brain Region Main olfactory bulb

Sub Region Glomerular layer

Side

View Ventral

Serial Number

Odor

Odorant Blend Male Mouse Urine (H-2K) Show Other Male Mouse Urine (H-2b) Show Other

Concentration 20% v/v of urine

Exposure Time 30 minutes

Exposure Frequency 3 min at 5 min intervals

Method c-fos in situ hybridization Show Other

Note Comparison of c-fos glomerular activity elicited by H-2b and H-2k male urine in the main olfactory bulbs of H-2d female mice. A, B, Color contour maps of the average evoked glomerular activity by either H-2b (A) or H-2k (B) urine. C, Map of the difference between H-2b and H-2k urine odor representations. p values for Mann-Whitney U tests are shown at each bin within the map. Color bar on the top of A shows the density of active glomeruli. Color bar on the top of C shows p values. The black border was drawn to indicate the limits of the Bonferoni Correction (p < 6 × 105) in C and used to overlay maps in A and B. D, Prediction of H-2 haplotype using H-2b and H-2k average odor maps in A and B, respectively, as templates. All individual maps used to construct averages in Figures 2 and 3 were tested. The graph shows the mean SSQ ratio at each urine odor concentration. A value <1 means the H-2 haplotype was correctly predicted from the individual map. Error bars plotted in one direction to avoid overlap represent SEM.

Authors Michele L. Schaefer, David A. Young, and Diego Restrepo

PubMed_ID 11264322

Map Picture

Scan Number Control

Scan Number Stimulate

Subject Count 4 for each odor

Image Resolution

Image Size

Primary Data

Behavioral Study